Mobile Information Kiosk in Mall using Wi-Fi

With the introduction of faster wireless networking technology, the emergence of wireless devices and mobile networking has opened up new business opportunity begin with e-commerce and now extends to mobile commerce (m-commerce). Basically, this Final Year Project proposed the development and implementation of the Mobile Information Kiosk in Mall using Wi-Fi- a mobile solution that satisfies shopping mall customer's need and busy working style. This report presents the research and study of the project as well as the outcome of the project that is Mobile Information Kiosk in Mall using Wi-Fi ' This ·project will address to the three problem statements which are larger shopping mall brings a larger number of shops, practicality of Web-site provided and last but not least network fees and charges. This followed by the objectives of the system that is to develop a system called " Mobile Information Kiosk in Mall using Wi-Fi" that can successfully aid visitors to find their desired retail stores or facilities or items in the shopping mall as well help visitors saving their time, money ,and effort. The scopes of the project are divided into two main parts that are to focus on information search and retrieval from system's database and also to focus on information display by providing the graphical mapping. Basically, the whole project will be applying a Waterfall Development Model due to its simplicity and requires linear progress. With this report, the basis for the research of this project will be underlined in detail, including the technologies as well the study of recent researches related to the study and also summarizing the result form the surveys that have been done through out t the whole period given . Finally the result of this project, will be in the mobile web application where user can easily access the system through Wi-Fi technology using their mobile phone or their Personal Digital Assistance (PDA

Mobile Information Kiosk in Mall using Wi-Fi

With the introduction of faster wireless networking technology, the emergence of wireless devices and mobile networking has opened up new business opportunity begin with e-commerce and now extends to mobile commerce (m-commerce). Basically, this Final Year Project proposed the development and implementation of the Mobile Information Kiosk in Mall using Wi-Fi- a mobile solution that satisfies shopping mall customer's need and busy working style. This report presents the research and study of the project as well as the outcome of the project that is Mobile Information Kiosk in Mall using Wi-Fi ' This ·project will address to the three problem statements which are larger shopping mall brings a larger number of shops, practicality of Web-site provided and last but not least network fees and charges. This followed by the objectives of the system that is to develop a system called " Mobile Information Kiosk in Mall using Wi-Fi" that can successfully aid visitors to find their desired retail stores or facilities or items in the shopping mall as well help visitors saving their time, money ,and effort. The scopes of the project are divided into two main parts that are to focus on information search and retrieval from system's database and also to focus on information display by providing the graphical mapping. Basically, the whole project will be applying a Waterfall Development Model due to its simplicity and requires linear progress. With this report, the basis for the research of this project will be underlined in detail, including the technologies as well the study of recent researches related to the study and also summarizing the result form the surveys that have been done through out t the whole period given . Finally the result of this project, will be in the mobile web application where user can easily access the system through Wi-Fi technology using their mobile phone or their Personal Digital Assistance (PDA

Flavokawain a induces apoptosis in MCF-7 and MDA-MB231 and inhibits the metastatic process in vitro

The kava-kava plant (Piper methsyticum) is traditionally known as the pacific elixir by the pacific islanders for its role in a wide range of biological activities. The extract of the roots of this plant contains a variety of interesting molecules including Flavokawain A and this molecule is known to have anti-cancer properties. Breast cancer is still one of the leading diagnosed cancers in women today. The metastatic process is also very pertinent in the progression of tumorigenesis

Flavokawain A induces apoptosis in MCF-7 and MDA-MB231 and inhibits the metastatic process in vitro

Introduction: The kava-kava plant (Piper methsyticum) is traditionally known as the pacific elixir by the pacific islanders for its role in a wide range of biological activities. The extract of the roots of this plant contains a variety of interesting molecules including Flavokawain A and this molecule is known to have anti-cancer properties. Breast cancer is still one of the leading diagnosed cancers in women today. The metastatic process is also very pertinent in the progression of tumorigenesis. Methods: MCF-7 and MDA-MB231 cells were treated with several concentrations of FKA. The apoptotic analysis was done through the MTT assay, BrdU assay, Annexin V analysis, cell cycle analysis, JC-1 mitochondrial dye, AO/PI dual staining, caspase 8/9 fluorometric assay, quantitative real time PCR and western blot. For the metastatic assays, the in vitro scratch assay, trans-well migration/invasion assay, HUVEC tube formation assay, ex vivo rat aortic ring assay, quantitative real time PCR and western blot were employed. Results: We have investigated the effects of FKA on the apoptotic and metastatic process in two breast cancer cell lines. FKA induces apoptosis in both MCF-7 and MDA-MB231 in a dose dependent manner through the intrinsic mitochondrial pathway. Additionally, FKA selectively induces a G2/M arrest in the cell cycle machinery of MDA-MB231 and G1 arrest in MCF-7. This suggests that FKA's anti-cancer activity is dependent on the p53 status. Moreover, FKA also halted the migration and invasion process in MDA-MB231. The similar effects can be seen in the inhibition of the angiogenesis process as well. Conclusions: FKA managed to induce apoptosis and inhibit the metastatic process in two breast cancer cell lines, in vitro. Overall, FKA may serve as a promising candidate in the search of a new anti-cancer drug especially in halting the metastatic process but further in vivo evidence is needed

Flavokawain A Induces Apoptosis in MCF-7 and MDAMB231 and Inhibits the Metastatic Process In Vitro

Introduction: The kava-kava plant (Piper methsyticum) is traditionally known as the pacific elixir by the pacific islanders for its role in a wide range of biological activities. The extract of the roots of this plant contains a variety of interesting molecules including Flavokawain A and this molecule is known to have anti-cancer properties. Breast cancer is still one of the leading diagnosed cancers in women today. The metastatic process is also very pertinent in the progression of tumorigenesis. Methods: MCF-7 and MDA-MB231 cells were treated with several concentrations of FKA. The apoptotic analysis was done through the MTT assay, BrdU assay, Annexin V analysis, cell cycle analysis, JC-1 mitochondrial dye, AO/PI dual staining, caspase 8/9 fluorometric assay, quantitative real time PCR and western blot. For the metastatic assays, the in vitro scratch assay, trans-well migration/invasion assay, HUVEC tube formation assay, ex vivo rat aortic ring assay, quantitative real time PCR and western blot were employed. Results: We have investigated the effects of FKA on the apoptotic and metastatic process in two breast cancer cell lines. FKA induces apoptosis in both MCF-7 and MDA-MB231 in a dose dependent manner through the intrinsic mitochondrial pathway. Additionally, FKA selectively induces a G2/M arrest in the cell cycle machinery of MDA-MB231 and G1 arrest in MCF-7. This suggests that FKA’s anti-cancer activity is dependent on the p53 status. Moreover, FKA also halted the migration and invasion process in MDA-MB231. The similar effects can be seen in the inhibition of the angiogenesis process as well. Conclusions: FKA managed to induce apoptosis and inhibit the metastatic process in two breast cancer cell lines, in vitro. Overall, FKA may serve as a promising candidate in the search of a new anti-cancer drug especially in halting the metastatic process but further in vivo evidence is needed

Flavokawain A inhibits the proliferation and induces apoptosis in both MCF-7 and MDA-MB231.

<p>A) The percentage of cell viability of MDA-MB231, MCF-7 and MCF-10A when treated with Flavokawain A for 72 hours.B) The percentage of BrdU incorporation in MCF-7 and MDA-MB231 after being treated with three different concentrations of flavokawain A for 48 hours.C) Histogram analysis of the cell cycle machinery in MCF-7 and MDA-MB231 after being treated with three different doses of flavokawain A for 12 and 24 hours.D) Histogram analysis of Annexin V/FITC in MDA-MB231 and MCF-7 after being treated with three different concentrations of flavokawain A after 48 hours. Data are expressed as mean ± S.E.M with p value< 0.05.</p

The values of IC₂₅, IC₅₀ and IC₇₅of FKA in MCF-7 and MDA-MB231 to be used in other assays.

<p>The values of IC₂₅, IC₅₀ and IC₇₅of FKA in MCF-7 and MDA-MB231 to be used in other assays.</p

qPCR analysis of metastasis-related genes; GLUT1, VEGF and ICAM in Flavokawain A-treated MDA-MB231 after 18 hours of treatment.

<p>The experiment was done in triplicates and the data are expressed as mean ± S.E.M.</p

Flavokawain A induces changes in the mitochondrial membrane potential and activates caspase 8 and 9 in MCF-7 and MDA-MB231.

<p>A) Bar chart and histogram analysis of the depolarization of mitochondrial membrane potential of MCF-7 and MDA-MB231 after treatment with three doses of flavokawain A for 48 hours.B) Detection of the activation of caspase 8 and 9 in both MCF-7 and MDA-MB231 after 48 hours of treatment with three concentrations of flavokawain A. Data are expressed as mean ± S.E.M..(p<0.05)</p

The accession number and sequence of the primers used in the quantitative real-time PCR assay.

<p>The accession number and sequence of the primers used in the quantitative real-time PCR assay.</p